Dor,
I am guessing you want to basically close the valve and pressure up against it to preform your test?
Certain types of valves, soft seated ball valves in particular, could be damaged by doing this.
Using a blind flange or some other expendable "plug" is the safest course of action. If that is just not an option, contact the valve manufacturer, and find out if their valve's seating/sealing mechanisim will handle the test pressure required. For certain types of valves, like certain plug valves, or metal seated valves, it may not really be a problem.
You should also take into consideration that the manufacturer will very likely void the warranty if your test pressure is above 1.1 times the valve's room temp MOP. So if you do damage the valve, you may very likely be replacing it out of pocket.
Best Regards,
VRF