I don't use the NASGRO database, although the materials I usually deal with are the normal aerospace aluminium alloys. As a general rule, the da/dN data that I use are generated in lab ambient conditions and no adjustment for actual operating environment is performed. In terms of civil aircraft, I can imagine that most operating environments are not totally dissimilar to the lab ambient conditions, and in any case, feedback from the fleet-wide scheduled inspection program will (should) detect any undesirable trends.
Early in my career I queried my then-employer about the fact that we did not take into account the (approx) +20C to -40C to +20C thermal cycle that external structural components, like wing skins, would experience. I never really got much of an explanation and did not pursue it. In any case, it can be argued that this thermal cycle is of little significance.
Short answer: No knockdown factor.