The best location depends on the oven's power source location. Nowadays, most feeds are vertically polarized and mounted on the short wall. If you are putting any lossy thing inside the oven, then it will affect the fields, and there won't be much of a resonance whereby one location is perfect compared to other locations. Hence just use anywhere on the far side wall. Either the middle or the top corner.
It sounds like you want to heat liquid via the tube. If this liquid is water, then the 0.5 inch hole will allow RF to propagate out the hole when the water flows and you may create a problem.
Waveguide cutoff is 0.5 * 11. 803/ 2.45 Ghz = 2.41 inches, but with water dielectric = 81 flowing thru, the cutoff dimension becomes 2.41/sqrt (81)= 0.27 inches.
Hence, it may be necessary to use smaller below cutoff holes and combine them (if the dielectric of your liquid flowing thru the tube is high like water).
If you have some 2.45 Ghz phones available to detect the leakage, it may give you a hint of just how bad the leakage is. With a potential leakage problem, having the hole nearer the corner of the oven may be best. i.e. the corner furthest away from the power source feed.
So how large is the heating need, slight or max?
kch