Yes. In the past I have used the gages both before and after plating. When we used them before plating, we first developed a correlation between the change in L1 and L2 reading before plating and after plating. The amount of change per plating thickness should be the same for both L1 and L2, because both check the functional surfaces of the threads and should not touch the thread crests. (Different gages are needed to check the thread crest diameter) Then we machined the threads so the L1 and L2 gages would engage by an "extra" amount before plating, something like 1/2 turn or 1 turn (I haven't worked with this in a while, so I don't remember the exact correlation to plating thickness).
We always checked again after plating to be sure.