Dear all members,
Hydrophobis membrane usually must be prewetted. And in every book or journal I have read, they always use ethanol.
I am confuse. Why must ethanol?
Why not methanol instead?
Since the membrane is hydrophobic, most permeable species probably interact with the membrane through london dispersion forces. Thus, ethanol has a higher molecular weight than methanol and will have more affinity for the hydrophobic membrane.