Jumacao
That's one of the problems with posting on a free Internet forum -- you don't really know. In the end, you need to make a decision based on sound engineering principles.
Part of your problem is that both answers might be correct, depending on the application and arrangement of the fans.
For example, my earlier reply was based on a "constant loss" scenario to use Waross' term. The intake is in the cleaner room and an exhaust fan is in the more contaminated room. Air flows into one room, through a damper, and then out the other room's exhaust. Flow is always from the clean to dirty room. There is no recirculation of air between the rooms. While this doesn't strictly "segregate" the flow between the rooms, it does ensure that the clean room doesn't get contaminated.
In a later post you asked about using the same air handling unit for both rooms. That is a slightly different question then "is it ok to use one supply fan and one exhaust fan and still keep the rooms segregated". An air handling unit cools/warms the air within a room and could be entirely separate from the intake and exhaust fans. Again, this would depend on your setup and what the limitations are for each room.
Having one intake fan, one air handling unit and one exhaust fan to handle the airflow for multiple rooms is not uncommon in the nuclear world (which is my experience base). The design engineer just needs to ensure that the design is adequate to keep each room within the desired temperature range and that the airflow is always in the desired direction.
Patricia Lougheed
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