That's easier to measure than to calculate. Detail calculations for higher isolation setups are not usually possible in todays antenna software.
Sample data results:
6-18 ghz 20 dB gain multi-beam antenna arrays, spaced 4 feet apart, one atop another will have 70 to 90 dB isolation just on their own and about 100 to 120 dB isolation if you try to block RF and surface currents between them. This is measured data I have on a real program from about 15 years ago.
Here is some estimated numbers of isolation for a pair of 15-25 dB horns one atop each other, just to give you my feeling for isolation levels versus difficulty (I've worked alot of isolation problems and have a good feel for the results);
60 dB isolation: easy to do, 3 foot separation, multi-path probably not an issue, unless you point at something within 50 feet? tree, etc.
80 dB isolation: Not too bad at ?6 feet separation, you may want to orient/move the antennas relative to each other, i.e. offset them, straight up and down is likely to be worse isolation than if they were say 3 feet vertical and 2 feet horizontally displaced.
100 dB isolation: difficult, multipath comes in
120 dB isolation: severe multipath problem. Example: One of our antenna array TX to single antenna Rx had 117 dB isolation worst case in the 6-18 ghz band, we received more energy from trees at 1100 feet away than we did from antenna to antenna coupling space 4 feet apart. Birds flying by would put blips on the recorded data when testing.
details on your setup and approx. frequency range would be nice.
kch