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ELISA 1

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AI2005

AI2005

Bioengineer
Joined
Jul 26, 2005
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2
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DE
What difference does it make using FCS(Foetal Calb Serum) or FBS(Foetal Bovine Serum) for blocking?.Does this affect in any way quality of the ELISA(Enzyme linked Immunosorbent Essay)?
Thanks in advance.
 
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There is no straight answer as such you find out by doing experiments but the logical answer to this question would be ! Yes there can some difference. This will be due to difference in property and composition. There may some cofactors in FCS, which are not in FBS. These kind of cofactors help in trigging the coupling reacton or they serve as affinity for antigen and antibody. It is very similar to the cascade reaction in Immunology.
 
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Thanks for this responds.I actually used both FCS and FBS in an experiment(ELISA) and the background signal seemed to alternate!.I was not very sure if it had something to do with me using FBS instead of FCS.As you said i also guest the best way to find out would be to carry out a series of test with both and see if any differences show up.
Thanks.
 
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