Fluorescent Tracer Particles Under Black Light
Fluorescent Tracer Particles Under Black Light
(OP)
Hi all,
Pretty sure this isn’t the most suitable forum for this question but so far, my best bet for an answer.
So I’ll just try to explain what is the final goal, because I’m not entirely sure what I should be looking for.
We are trying to follow a stream of nebulized liquid through a transparent model representing the human upper airway tree.
The idea is to "flood" the model with “black light” and use a solution of the liquid with a fluorescent dye or tracer particles, of some sort, so that only the nebulized stream will be visible.
I was hoping to find something that is non-toxic and preferably bio-compatible, so if anyone can nudge me in the right direction, that would be great!
(I’m searching the web for an answer in parallel to this thread)
Pretty sure this isn’t the most suitable forum for this question but so far, my best bet for an answer.
So I’ll just try to explain what is the final goal, because I’m not entirely sure what I should be looking for.
We are trying to follow a stream of nebulized liquid through a transparent model representing the human upper airway tree.
The idea is to "flood" the model with “black light” and use a solution of the liquid with a fluorescent dye or tracer particles, of some sort, so that only the nebulized stream will be visible.
I was hoping to find something that is non-toxic and preferably bio-compatible, so if anyone can nudge me in the right direction, that would be great!
(I’m searching the web for an answer in parallel to this thread)
RE: Fluorescent Tracer Particles Under Black Light
It fluoresces nicely,
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P.E. Metallurgy, Plymouth Tube
RE: Fluorescent Tracer Particles Under Black Light
As the mist of the nebulized liquid is very fine, "flooding" the model with visible light will impair our ability to see the stream (my assumption), this is why I'm looking for something that might work under the "Blacklight" spectrum.
RE: Fluorescent Tracer Particles Under Black Light
I have used flourescein for inspection inside of food processing tanks. I used standard black lights and got very good visibility.
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P.E. Metallurgy, Plymouth Tube
RE: Fluorescent Tracer Particles Under Black Light
RE: Fluorescent Tracer Particles Under Black Light
I've checked on wikipedia for the wave length for optimal emission, but in case i wouldn't find the correct lamp...
RE: Fluorescent Tracer Particles Under Black Light
We used flourescien because it was food grade (actually injectable) and if we left traces in the tank there would't be contamination issues (unless you are worried that your beer glows).
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P.E. Metallurgy, Plymouth Tube
RE: Fluorescent Tracer Particles Under Black Light
If there is a mist, however, you may still not be able to see anything, not due to wavelengths but due to scattering.
RE: Fluorescent Tracer Particles Under Black Light
Just in case -
The lamp I used was something like the following:
https://www.uv-light.co.uk/blacklight/hand-held/35...
I don't have access to the instrument anymore to dig up it's spec sheet but what I remember is that the lamps match with some of the local maxima of the absorption spectra in the 400nm range.
RE: Fluorescent Tracer Particles Under Black Light
I will go for the lamp with the specified wavelength, just to be sure that there will be the maximum amount of excitation from the nebulized stream.
About the ability to se the glow from the nebulized stream, I hope that I will be able to see it with the naked eye, as we used a 2.26W, 532nm green laser to outline a bolus of the nebulized solution w/o the fluorescein, we were able to see it with the naked eye (see attached photo).
But in case you are right, and the glow will be barely visible, we plan to use a specialized camera to filter the photo/video.
RE: Fluorescent Tracer Particles Under Black Light
RE: Fluorescent Tracer Particles Under Black Light