Image Analysis

[Shahram56]

I have a couple of AFM images of Quantum DOTs and need to process them. Here is the idea? I have the JPG or TIFF file with some dot on the image and can transfer it to gray scale and make a threshhold and then count the number of dots and the Ic an find the density. I also need to find the distribution of size? is there any softwar that can Help me?

 

[MacGyverS2000]

If you want to simply do all of that by hand, grab something like Paint Shop Pro Trial and go to it... free. Distribution of size is another matter, but it's doable... one way (although very time consuming) would be to crop each dot and count the number of pixels above the threshold (look at the histogram data)... you know the total image size, so you can get dot size relative to all other dots in the image by looking at their relative pixel counts.

If you want something automated, that's going to cost you a bit more. There are programs that do just as you described in the scientific community, but they charge an arm and a leg for it (they figure most scientists are too busy to implement such simplistic software themselves, so they charge accordingly).

The more steps you can do yourself, the cheaper it is.


Dan
Owner

http://www.Hi-TecDesigns.com

 

[IRstuff]

You could try Matlab and its Image Processing toolbox.

Alternately, you can try Imagetool, which is a free download and has some capabilities along those lines:

http://ddsdx.uthscsa.edu/dig/itdesc.html

TTFN

 

[Shahram56]

Thanks IR Stuff.
I have the free software I think I need to check the image processing toolbox of MatLab.
Sean

 

[m777182]

I had a similar problem: I made a BMP format file(of 5,2 MBytes) and read it as a string of integer values (0...255) into a variable that was declared in four dimensions, for red, blue, green and white (as a sume of the previous three)and with a lenght of the number of pixels on your image. Then you can make any statistic that you wish. To make a discrimination of 8 adjacent points of each particular point I used a minimum gradient threshold to separate significant differences among points. If you adjust the threshold limit you will separate them very efficiently.
m777182

 

[evdsande]

Try Image Pro from Media Cybernetics, a very good image analysis package, it has all the image analysis tools you need. Or you could go for the free ImageJ which has the same features but lacks the slick user interface.

 

[Shahram56]

Thanks,

I am using ImageJ. It is giving me reasonable results.