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  1. Jigneshpadia2002

    Cooling capacity of chiller and heat load calculation

    Can I please ask one question! I have a situation. I need to make sure that a particular cooling unit / Equipment is suitable for a process or not. I am bit new with this type of assignments! I am sure it won't be very challenging for the experts but I really wish to get some help with this...
  2. Jigneshpadia2002

    Whats the standard protocol to treat biopharmaceutical waste water?

    Hi, I am curious to know the waste water treatment for biopharmaceuticals containing Antibiotics. In our company we use CIP-100 (its a caustic detergent) to kill the bugs before dumping to the drain but I don't recall any specific treatment given for the cultures containing antibiotics...
  3. Jigneshpadia2002

    Relationship of backpressure and flow!

    Hi ! I was doing one experiment on Tangential Flow Filtration membrane and I was confused by the flow outputs with and without pressure. My assumption was For a close system: Input = Output Feed flowrate = Permeate flowrate + Retentate flowrate When I started the experiment without any...
  4. Jigneshpadia2002

    Heat Transfer in Bioreactor!

    Hi Everyone! I am interested in calculating the heat transfer efficiency of bioreactors. I have few basic questions and I am hoping them someone can help me understand and clarify my doubts. 1. How to calculate the heat generation due to Impeller RPM. I have done some homework and found that...
  5. Jigneshpadia2002

    Filters for Clean Steam Generator

    Hi ! Can any one explain me abou the opration and function of Clean Steam filters. How to validate the system and the filters. How to perform the integrity on the SS filters? Thanks a lot, Jignesh
  6. Jigneshpadia2002

    Integrity Testing of Steam Filters

    Can anyone tell me the protocol or method for integrity testing of steam filters. Thanks
  7. Jigneshpadia2002

    Recent 483s on filter validation

    Can any one help me to find online resource to findout about recent 483s for filter validation. I tried FDA websit, warning letters section but was unable to get any specific case study. Please let me know if anyone came across or know where to find such informations. Thanks a lot in advance!
  8. Jigneshpadia2002

    What is the Validation requirement for HVAC filters

    Can anyone provide me brief information on FDA validation process for HVAC filters?
  9. Jigneshpadia2002

    Cost of running Utlilities

    Can Anyone give some idea about the cost of running Air, Steam and WFI in a mid size Biotech or Pharma plant? What is the typical consumption of WFI, Air & Steam? Say in a company like Glaxo, Pfizer or Elilly!
  10. Jigneshpadia2002

    Dimensional Analysis for Impeller

    Hi there, I am working on a small scale spinner system for growing mammalian cells. Spinners are labscale instruments which are very different in terms of geometry of the impeller comapre to large scale or pilot scale bioreactors. Problem with this spinners is that there is no scale-up...
  11. Jigneshpadia2002

    Scale up of Centrifuge

    Hi Everyone, I am a biotechnologist! I am working in a process development area. I am very much keen to learn about the engineering principles and rule of thumbs of scale-up. Recently during my literature survery I came across a strange Dimensionless number for centrifuge scale-up. During my...
  12. Jigneshpadia2002

    membrane filtration handbook

    www.gewater.com/pdf/ 1229223-%20Lit-%20Membrane%20Filtration%20Handbook.pdf
  13. Jigneshpadia2002

    Cell lysate clarification

    Can I use depth filter for E.coli cell lysate clarification? Any suggestions on NFF options. I already have the TFF & centrifuge options with me but I am very curious to know about the NFF ( Depth filters )
  14. Jigneshpadia2002

    Pump for Chromatography system

    Can anyone give more information about the chromatography pumps. I am intending these pumps for IonExchange chromatography. Small scale and medium scale. Colimn dia around 1.1 cm to 3 cm Linear flow rate around: 300 cm/h Thanks a lot in advance.
  15. Jigneshpadia2002

    DOE(Design of Experiments

    Can anyone please help me to understand how I can design the Taguchi's DOE (Design of Experiment). I am not aware about the rules. I came across this equation Ln(2^K) Where n= number of experiments 2 = levels per factor (i.e. High & Low ) k = number of factors But how to find N I have seen...
  16. Jigneshpadia2002

    Taguchi's L8 Orthogonal array

    Can anyone please help me to understand how I can design the Taguchi's DOE (Design of Experiment). I am not aware about the rules. I came across this equation Ln(2^K) Where n= number of experiments 2 = levels per factor (i.e. High & Low ) k = number of factors But how to find N I have seen...
  17. Jigneshpadia2002

    About FE

    Hi There, I have gone through the thread written on FE by waskillywabbit but I have some different problems ! I am a graduate in Microbiology and than I did Masters in Biochemical Engineering. I really liked it! It is so fun to be an Engineer but at the same time it is difficult and it brings...
  18. Jigneshpadia2002

    which is the common cell line used in Industry ?

    I am developing a cell culture laboratory and we are looking to get a cell line which can be used as a bench mark. So can anyone give an idea which one is the most commom. I found that CHO cell line is the one but is it possible to get it in a serum free vials/ seed. Thanks a lot,
  19. Jigneshpadia2002

    What is general chemical Formula of CHO cell

    Hi guys I am doing mass balance for a fermentation process and I am interested in knowing the chemical formula of CHO cell. Please let me know if anyone has done similar mass balance. Thanks a lot in advance
  20. Jigneshpadia2002

    What is PID

    What is PID ? Can anyone provide basic information on functioning of PID ? Thanks in advance

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