Can I please ask one question!
I have a situation. I need to make sure that a particular cooling unit / Equipment is suitable for a process or not. I am bit new with this type of assignments! I am sure it won't be very challenging for the experts but I really wish to get some help with this...
Hi,
I am curious to know the waste water treatment for biopharmaceuticals containing Antibiotics.
In our company we use CIP-100 (its a caustic detergent) to kill the bugs before dumping to the drain but I don't recall any specific treatment given for the cultures containing antibiotics...
Hi !
I was doing one experiment on Tangential Flow Filtration membrane and I was confused by the flow outputs with and without pressure.
My assumption was
For a close system: Input = Output
Feed flowrate = Permeate flowrate + Retentate flowrate
When I started the experiment without any...
Hi Everyone!
I am interested in calculating the heat transfer efficiency of bioreactors. I have few basic questions and I am hoping them someone can help me understand and clarify my doubts.
1. How to calculate the heat generation due to Impeller RPM.
I have done some homework and found that...
Hi !
Can any one explain me abou the opration and function of Clean Steam filters. How to validate the system and the filters. How to perform the integrity on the SS filters?
Thanks a lot,
Jignesh
Can any one help me to find online resource to findout about recent 483s for filter validation. I tried FDA websit, warning letters section but was unable to get any specific case study.
Please let me know if anyone came across or know where to find such informations.
Thanks a lot in advance!
Can Anyone give some idea about the cost of running Air, Steam and WFI in a mid size Biotech or Pharma plant?
What is the typical consumption of WFI, Air & Steam? Say in a company like Glaxo, Pfizer or Elilly!
Hi there,
I am working on a small scale spinner system for growing mammalian cells. Spinners are labscale instruments which are very different in terms of geometry of the impeller comapre to large scale or pilot scale bioreactors. Problem with this spinners is that there is no scale-up...
Hi Everyone,
I am a biotechnologist! I am working in a process development area. I am very much keen to learn about the engineering principles and rule of thumbs of scale-up. Recently during my literature survery I came across a strange Dimensionless number for centrifuge scale-up.
During my...
Can I use depth filter for E.coli cell lysate clarification? Any suggestions on NFF options. I already have the TFF & centrifuge options with me but I am very curious to know about the NFF ( Depth filters )
Can anyone give more information about the chromatography pumps.
I am intending these pumps for IonExchange chromatography. Small scale and medium scale.
Colimn dia around 1.1 cm to 3 cm
Linear flow rate around: 300 cm/h
Thanks a lot in advance.
Can anyone please help me to understand how I can design the Taguchi's DOE (Design of Experiment). I am not aware about the rules.
I came across this equation
Ln(2^K)
Where n= number of experiments
2 = levels per factor (i.e. High & Low )
k = number of factors
But how to find N
I have seen...
Can anyone please help me to understand how I can design the Taguchi's DOE (Design of Experiment). I am not aware about the rules.
I came across this equation
Ln(2^K)
Where n= number of experiments
2 = levels per factor (i.e. High & Low )
k = number of factors
But how to find N
I have seen...
Hi There,
I have gone through the thread written on FE by waskillywabbit but I have some different problems !
I am a graduate in Microbiology and than I did Masters in Biochemical Engineering. I really liked it! It is so fun to be an Engineer but at the same time it is difficult and it brings...
I am developing a cell culture laboratory and we are looking to get a cell line which can be used as a bench mark. So can anyone give an idea which one is the most commom. I found that CHO cell line is the one but is it possible to get it in a serum free vials/ seed.
Thanks a lot,
Hi guys I am doing mass balance for a fermentation process and I am interested in knowing the chemical formula of CHO cell. Please let me know if anyone has done similar mass balance.
Thanks a lot in advance
